Application

Detect Fibronectin using this Anti-Fibronectin Antibody, cellular, clone DH1 validated for use in WB, IH.

Immunoblotting at 1:1,000

Indirect immunohistochemistry at 1:200-1:400

A 1-hour fixation with 3-3.5% paraformaldehyde in 0.1 M phosphate buffer pH 7.4 is suggested. Shows some reactivity on paraffin sections depending on fixation conditions and time (Latvala et al., 1996).

Final working dilutions must be determined by end user.

Immunogen

Epitope: cellular

Purified fibronectin from Human A8387 fibrosarcoma cells.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Format: Purified

In PBS with 1% BSA and 0.1% sodium azide.

Specificity

Specific for the extra domain of cellular fibronectin (EDA sequence) and thus recognizes only the cellular fibronectin.